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Therefore, we used the Michaelis-Menten equation to determine the k cat and K m values at the optimal pH of the remaining GUS enzymes analysed to generate a complete set of kinetic parameters suitable for comparison (Table 1 and Figs S5, S6, and S7)
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Unless otherwise indicated, the expression of Ate1 was achieved by adding 2% galactose at 30 Β°C for 5β6 h, which is not expected to trigger major (more than 50%) cell death events by the overexpression itself [9]
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