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To understand whether the GLP-1–MK-801 conjugate activates distinct neural circuitries, we used light-sheet microscopy and cFOS immunohistochemistry to obtain quantitative 3D whole-brain activity signatures in response to GLP-1–MK-801 and semaglutide dosing (Fig
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DNA was stained with 7-aminoactinomycin D (7-AAD) and cells were analyzed using a FACSCalibur4C (Becton Dickinson) with the FL-1 channel (530 ± 30 nm band pass) for BrdU and the FL-3 channel (670 nm long pass) for 7-ADD
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