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Understanding Concentrations The concentration of your reconstituted peptide is determined by a simple formula: Concentration (mcg/mL) = Total Peptide (mcg) / Volume of Bac Water (mL) Example 1: 5 mg Vial with 2 mL Bac Water Convert milligrams to micrograms: 5 mg = 5,000 mcg Divide by volume: 5,000 mcg / 2 mL = 2,500 mcg/mL For a 250 mcg dose: 250 / 2,500 = 0.1 mL = 10 units on an insulin syringe Example 2: 10 mg Vial with 2 mL Bac Water 10 mg = 10,000 mcg 10,000 mcg / 2 mL = 5,000 mcg/mL For a 500 mcg dose: 500 / 5,000 = 0.1 mL = 10 units Example 3: 5 mg Vial with 1 mL Bac Water 5 mg = 5,000 mcg 5,000 mcg / 1 mL = 5,000 mcg/mL For a 250 mcg dose: 250 / 5,000 = 0.05 mL = 5 units Notice how the volume of bac water you choose directly affects how many units you need to draw per dose

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