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Then imaging was performed on an Axio Observer Z1 microscope (Carl Zeiss) equipped with a 100×, 1.49 NA objective, an electron-multiplying (EM) charge-coupled device (CCD) camera (Andor iXon+ DU-897D), and the 488/561 nm lines of a Sapphire CW CDRH USB Laser System attached to a spinning disk confocal scan head (Yokogawa CSU-X1 Spinning Disk Unit)
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