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coli strains were grown in liquid or solid Luria broth (LB) medium, supplemented with appropriate antibiotics: carbenicillin (100 μg ml −1 ), kanamycin (50 μg ml −1 ) or chloramphenicol (34 μg ml −1 )

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A1AT also has an effect on B lymphocytes, reducing their proliferation and function, as demonstrated in murine models of autoimmune diseases mediated by autoantibodies, such as rheumatoid arthritis, indicating a possible exploitation of A1AT as a therapeutic agent in autoimmune diseases (59) As for the effect on T cells, also the effect of A1AT on B lymphocytes seems to be indirect, mediated by the alterations in the extracellular milieu ( Figure 2 )